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EMERGENT LIFE SCIENCES RESEARCH - Vol 9, Issue 2, Published on 31, December 2023

Pages: 147-158
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Host extracts, sugars and amino acids concentrations enhanced growth of Macrophomina phaseolina

Author: M. Surya Prakash Reddy, M. Santha Lakshmi Prasad, Ashwini Kumar, K. T. Ramya, J. Jawaharlal

Category: Research Article


Macrophomina phaseolina is a soil inhabitant and it is pathogenic to a wide range of host crops. In the present investigation, M. phaseolina was grown in an enriched medium using semi mature stage of plant parts i.e. capsule/pod/head, leaf, stem, root Viz., castor, safflower, mustard, sunflower, groundnut, soybean, sesame crop parts were used at different concentrations. Furthermore, various carbon and amino acid sources were assessed at different concentrations to investigate their impact on the fungus growth under controlled in-vitro conditions. Results showed oil seed crop extracts significantly enhanced mycelial growth was recorded at all concentrations except castor stem extract which recorded the lowest mycelial growth over control, therefore carbon sources @ 250 ppm and 500 ppm of galactose, mannose, inositol, glucose, fructose, dextrose, and mannitol significantly influenced mycelial growth of M. phaseolina. All eight carbon sources have shown almost similar trends of mycelial development and amino acid sources @ 2500 ppm, 5000 ppm, 7500, and 10000 ppm showed maximum mycelial growth was recorded at 2500 ppm, 5000 ppm, 7500 ppm as increasing concentration reduced growth of mycelium however L-Lysine monohydrate recorded lowest mycelial growth at all concentrations.

Keywords: amino acid sources, carbon, Macrophomina phaseolina, oilseed crops