<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.2d1 20170631//EN" "JATS-journalpublishing1.dtd">
      <JournalTitle>Emergent Life Sciences Research</JournalTitle>
      <PISSN>2395-6658 (</PISSN>
      <EISSN>) 2395-664X (Print)</EISSN>
      <ArticleType>Research Article</ArticleType>
      <ArticleTitle>Molecular diversity analysis of rice (Oryza sativa L.) genotypes using SSR and RAPD marker</ArticleTitle>
          <FirstName>BALDANIYA VIPULKUMAR</FirstName>
      <Abstract>Simple sequence repeat (SSR) and random amplified polymorphic DNA (RAPD) molecular markers were used for detecting the genetic variability of 20 rice genotypes using 79 SSRs and 30 RAPD primers. Among primers used, a set of 16 SSR and 14 RAPD markers showed polymorphism, and banding patterns were scored as 1 (present) or 0 (absent) in the datasheet which was further analysed by using Jaccard’s similarity coefficient and SAHN clustering. The level of polymorphism among the rice genotypes was evaluated from the number of alleles, PIC value, and heterozygosity for individual 16 SSR and 14 RAPD markers. A total of 36 polymorphic loci were found in 16 polymorphic SSR markers and 77 polymorphic loci were observed in 14 polymorphic RAPD markers. The number of alleles per locus generated varied from 2 (RM447, RM21, RM171, RM237, RM25, RM283, RM510, RM259, RM334, RM433, RM489, and RM212) to 3 (RM263, RM152, RM413, and RM3331) in 16 SSRs markers and 3 (OPR-05) to 9 (OPR-02 and OPC-06) in 14 RAPD. In SSRs, the PIC value varied from 0.30 (RM447) to 0.58 (RM152) with an average of 0.38 per locus and the average value of heterozygosity (0.49). RAPD analysis showed an average PIC value of 0.78. Based on the information generated, the 20 rice genotypes were grouped into two main clusters in both the analysis of the marker. In SSR markers analysis, cluster I comprised of 4 genotypes and cluster II comprised of 16 genotypes and in RAPD cluster I was shown 2 genotypes and cluster II show 18 genotypes.</Abstract>
      <Keywords>Rice,Molecular diversity,SSR markers,RAPD markers,Polymorphism</Keywords>
        <Abstract>https://emergentresearch.org/ubijournal-v1copy/journals/abstract.php?article_id=14116&amp;title=Molecular diversity analysis of rice (Oryza sativa L.) genotypes using SSR and RAPD marker</Abstract>