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      <JournalTitle>Emergent Life Sciences Research</JournalTitle>
      <PISSN>2395-6658 (</PISSN>
      <EISSN>) 2395-664X (Print)</EISSN>
      <ArticleType>Research Article</ArticleType>
      <ArticleTitle>Evaluation of various substrates for mass production and shelf life of Trichoderma asperellum</ArticleTitle>
      <Abstract>Species belonging to the genus Trichoderma are considered as one of the most potential bio-control agents which can be readily collected from soil and found effective against various diseases including plant parasitic nematodes. Trichoderma species are commonly found in natural soil, decaying organic plant material and wood. They belong to the order Hypocreales within the Ascomycota phylum and are classified as imperfect fungi. Formulation and shelf-life are of prime importance for the commercial use of any bio-control agent. Present study was carried out to evaluate different substrates for mass production and shelf-life of Trichoderma asperellum (FbMi4 and FbMi6) isolates. The initial mean spores of T. asperellum FbMi4 at 30, 40 and 50 ml/100 g carrier on 1st day was 226×106, 255×106 and 291×106 spores/ml, respectively. It gradually declined and at 60 days of storage the population came down to 149×106, 169×106 and 192×106 spores/ml which in terms of reduction in viability was 14.9, 14.2 and 14.6%, respectively. Similar trends were also recorded in T. asperellum FbMi6 and sorghum grains talc based formulations. After 45 days of storage, neem cake was the organic amendment that produced the greatest amount of spores in T. asperellum FbMi4 and T. asperellum FbMi6 as compared to potato dextrose broth. Sufficient number of spores was viable even after 60 days of storage in the formulation indicating that the formulations can be stored further for more time.</Abstract>
      <Keywords>Formulation,fungal bio-agent,mass production,shelf-life,Trichoderma asperellum</Keywords>
        <Abstract>https://emergentresearch.org/ubijournal-v1copy/journals/abstract.php?article_id=14839&amp;title=Evaluation of various substrates for mass production and shelf life of Trichoderma asperellum</Abstract>