Comparative study on lactic acid production of different lactic acid bacteria through RP-HPLC method
DOI:
https://doi.org/10.7324/ELSR.2017.311117Keywords:
biogas, bio-resources, fallen leaves, methaneAbstract
In this study, a simple and reliable HPLC procedure has been developed for determination of lactic acid (LA) in liquid bacterial cultures. The lactic acid concentration is used as a criterion for strain selection. Eight LAB strains have been isolated from silage inoculants and food grade rice seeds. All strains showed the potential to produce more than 0.1 g/L LA, three strains produced LA above 0.8g/L, and one strain has the potential to produce 2.94g/L LA.
References
[1] C. Nordqvist (2004). Lactic acid bacteria - their uses in food. Medical News Today. Retrieved from http:// www.medicalnewstoday.com/releases/14023.php
[2] F. Leroy, L. De Vuyst (2004). Lactic acid bacteria as functional starter cultures for the food fermentation industry. Trends Food Sci. Technol., 15: 67-78.
[3] S. A. Abdullah, M. M. Osman (2010). Isolation and Identification of Lactic Acid Bacteria from Raw Cow Milk, White Cheese and Rob in Sudan. Pakistan Journal of Nutrition, 9:1203-1206.
[4] L. Axelsson (2004). Lactic acid bacteria: classification and physiology. In: S. Salminen, A. von Wright, A. Ouwehand, ed. Lactic acid bacteria: microbiological and functional aspects. Marcel Dekker Inc, New York, pp. 1-66.
[5] D. Ercolini, G. Moschetti, G. Blaiotta, S. Coppola (2001). Behavior of variable V3 region from 16S rDNA of lactic acid bacteria in denaturing gradient gel electrophoresis. Curr. Microbiol., 42: 199-202.
[6] J. M. Jay (2000). Fermentation and fermented dairy products. Modern food microbiology. 6th edition. Gaithersburg, USA: An Aspen Publication, Aspen Publishers, Inc., pp. 113-130.
[7] W. H. Holzapfel, P. Haberer, R. Geisen, J. Björkroth, U. Schillinger (2001). Taxonomy and important features of probiotic microorganisms in food nutrition. Am. J. Clin. Nutr., 73: 65-73.
[8] A. Sofyan, M. Angwar, H. Herdian, E. Damayanti, L. Istiqomah, A. Febrisiantosa, H. Julendra et al. (2012). Performance Enhancement and Immunity Profile of Broiler Treated Feed Additive Containing Lactic Acid Bacteria and Ganoderma lucidum. Med. Pet., 35: 201-206.
[9] M. A. K. Torshizi, S. H. Rahimi, N. Mogjani, S. Esmaeilkhanian, J. L. Grimes (2008). Screening of indigenous strains of lactic acid bacteria for development of a probiotic for poultry. Asian-Australian J. Anim. Sci., 21: 1495-1500
[10] K. Angmo, A. Kumari, Savitri, T. C. Bhalla (2016). Probiotic characterization of lactic acid bacteria isolated from fermented foods and beverage of Ladakh. LWT - Food Sci.Technol., 66: 428-435.
[11] J. L. Ellis, A. Bannink, I.K. Hindrichsen, R. D. Kinley, W. F. Pellikaan, N. Milora, J. Dijkstra (2016). The effect of lactic acid bacteria included as a probiotic or silage inoculant on in vitro rumen digestibility, total gas and methane production. Anim. Feed Sci. Tech., 211: 61-74.
[12] N. Limanska, T. Ivanytsia, O. Basiul, K. Krylova, V. Biscolla, J. Chobert, V. Ivanytsia et al. (2013). Effect of Lactobacillus plantarum on germination and growth of tomato seedlings. Acta Physiol. Plant., 35: 1587-1595.
[13] L. Istiqomah, S. N. Hayati, E. Damayanti, H. Julendra, A. A. Sakti, T. Untari (2013). Performance and Meat Quality of Broilers Infected with Escherichia coli and Administered with Bio Additive, Probiotic, and Antibiotic. Med. Pet., 36: 14-20,
[14] M. Nouri, F. Rahbarizadeh, D. Akhmadvand, F. Moosakhani, E. Sadeqzadeh, S. Lavasani, V. K. Vishteh (2010). Inhibitory effects of Lactobacilus salivarius and Lactobacillus crispatus isolated from chicken gastrointestinal tract on Salmonella enteritidis and Escherichia coli growth. Iranian J. Biotechnol., 8: 32-37.
[15] S. N. Jannah, A. Dinoto, K. G. Wiryawan, I. Rusmana (2014). Characteristics of Lactic Acid Bacteria Isolated from Gastrointestinal Tract of Cemani Chicken and Their Potential Use as Probiotics. J. Anim. Sci. Technol., 37: 182-189.
[16] F. Manini, M.C. Casiraghi, K. Poutanen, M. Brasca, D. Erba, C. Plumed-Ferrer (2016). Characterization of lactic acid bacteria isolated from wheat bran sourdough. LWT - Food Sci. Technol., 66: 275-283.
[17] T. C. Chung, L. Axelsson, S..E. Lindgren, W. J. Dobrogosz (1989). In vitro studies on reuterin synthesis by Lactobacillus reuteri. Microbial Ecol. Health Dis., 2: 137-144.
[18] P. A. Vanderbergh (1993). Lactic acid bacteria, their metabolic products and interference with microbial growth. FEMS Microbiol. Rev., 12: 221-237.
[19] L. De Vuyst, E. J.Vandamme (1994). Antimicrobial potential of lactic acid bacteria. In. Bacteriocins of lactic acid bacteria. 1st ed. Blackie Academic and Professional, Chapman and Hall, Glasgow, pp 91-142.
[20] A. S. Naidu, W. R. Bidlack, R. A. Clemens (1999). Probiotic Spectra of Lactic Acid Bacteria (LAB). Crit. Rev. Food Sci. Nutr., 39: 113-126.
[21] F. Djadouni, M. Kihal (2012). Antimicrobial activity of lactic acid bacteria and then spectrum of their biopeptides against spoiling germs in foods. Braz. Arch. Biol. Techn., 55: 435-443.
[22] S. Lindgren, W.J Dobrogosz (1990). Antagonistic activities of lactic acid bacteria in food and feed fermentations. FEMS Microbiol. Rev., 87:149-164
[23] Y. Kuo, Shuo-Fu Yuan, Chun-An Wang, Yin-Jung Huang, Gia-Luen Guo, Wen-Song Hwang (2015). Production of optically pure L -lactic acid from lignocellulosic hydrolysate by using a newly isolated and D -lactate dehydrogenase gene-deficient Lactobacillus paracasei strain. Bioresource Technol., 198: 651-657.
[24] D. Asami, Yun-Jeong Hong, Diane M. Barrett, Alyson E. Mitchell (2003). Comparison of the Total Phenolic and Ascorbic Acid Content of Freeze-Dried and Air-Dried Marionberry, Strawberry, and Corn Grown Using Conventional, Organic, and Sustainable Agricultural Practices. J. Agric. Food Chem., 51:1237-1241.
[25] Y. Hor, Min Tze Liong (2014).Use of extracellular extracts of lactic acid bacteria and bifidobacteria for the inhibition of dermatological pathogen Staphylococcus aureus. Dermatologica Sinica, 32: 141-147.
[26] R. Georgieva, P. Koleva, D. Nikolova, D. Yankov, S. Danova (2009). Growth parameters of probiotic strain Lactobacillus plantarum, isolated from traditional white cheese. Biotechnol. & Biotechnol. Eq., 23: 862-865
[27] S. Nakano, Ch. U. Ugwu and Y. Tokiwa (2012). Efficient production of D -(-)-lactic acid from broken rice by Lactobacillus delbrueckii using Ca(OH) 2 as a neutralizing agent. Bioresour. Technol., 104: 791–794.
[28] S. Te'llez-Luis, A. B. Moldes, M. Va´zquez and J. L. Alonso (2003). Alternative media for lactic acid production by Lactobacillus delbrueckii NRRL B-445. Food Bioprod. Process, 81: 250-256.
[29] K. Hofvendahl and B. Hahn-Hagerdal (2000). Factors affecting the fermentative lactic acid production from renewable resources. Enzyme Microb Technol., 26: 87-107.
[30] O. Elferink, S. J. W. H., J. Krooneman, J. C. Gottschal, S. F. Spoelstra, F. Faber, F. Driehuis (2001). Anaerobic conversion of lactic acid to acetic acid and 1,2-propanediol by Lactobacillus buchneri. Appl. Environ. Microbiol., 67: 125-132.
[31] C. Zhang, M. J. Brandt, C. Schwab, M. G. Gänzle (2010). Propionic acid production by cofermentation of Lactobacillus buchneri and Lactobacillus diolivorans in sourdough. Food Microbiol., 27: 390-395.
Downloads
Published
Issue
Section
License
Copyright (c) 2017 Author (s)

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Copyright © The Author(s). This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND 4.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original author(s) and source are properly credited, and the work is not modified or adapted.
