Analytical Method Development of Amodiaquine Hydochloride in Marketed Formulation by UV Visible Spectrophotometry
Keywords:
amodiaquine hydrochloride, first order derivative, UV Spectrophotometer, zero order derivativeAbstract
In the present study, two simple, precise and economical UV methods have been developed for the estimation of Amodiaquine Hydrochloride in bulk formulation using methanol. Method A involved the measurement of UV absorbance in zero order derivative at 343 nm, while Method B involved the measurement of UV absorbance in first order derivative at 217.5 nm. Both methods, A and B followed Beer-Lambert’s law with the drug in the concentration range of 10-70 μg/ml and correlation coefficient (r2) 0.9998 and 0.9997, respectively. The limit of detection for zero order and first order was found to be 0.032 and 2.716 respectively. The limit of quantification for zero order and first order was found to be 0.097, 8.231 respectively. Outcomes of the experiments were found appropriate on statistical confirmation. Hence, suggested procedures can be effectively utilized for the assessment of Amodiaquine hydrochloride in regular analytical studies.
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